CST, 94063T, Spred1 (D6D8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPRED1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Spred1 (D6D8B) Rabbit Monoclonal Antibody (CST #94063) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Spred1 (D6D8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Spred1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln281 of human Spred1 protein. Background Spred1 belongs to the Spred family functioning as sprouty-related suppressor of Ras signaling down stream of growth receptor signaling (1,2). The protein has a N-terminal EVH1 (Ena/VASP homology-1) domain, followed by a KBD (c-Kit- binding domain) and a C-terminal SPR (Sprouty) domain. The C-terminal SPR domain functions to translocate Spred1 to the membrane following growth factor stimulation (1,3). The N-terminal EVH1 domain interacts with neurofibromin to bring the later to the membrane proximal signaling complex for down regulation of Ras signaling (4,5). Mutations of Spred1 are the causes of Legius Syndrome (6,7). Spred1 expression inhibits tumor cell motility and metastasis, and down regulation has been found in hepatocarcinoma and myeloblastic leukemia (8-10). Alternate Names EVH1/Sprouty domain containing protein; FLJ33903; hSpred1; LGSS; NFLS; PPP1R147; protein phosphatase 1, regulatory subunit 147; SPRE1; Spred-1; SPRED1; sprouty related EVH1 domain containing 1; sprouty-related, EVH1 domain containing 1; Sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG