CST, 94080S, SP3 Antibody

Polyclonal Antibody for studying SP3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SP3 Antibody (CST #94080) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SP3 Antibody recognizes endogenous levels of total SP3 protein. The antigenic peptide sequence is 100% conserved among the 6 isoforms of SP3 reported in Uniprot. This includes splice variants and isoforms generated by alternative translation initiation. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp760 of human SP3 protein. Antibodies are purified by peptide affinity chromatography. Background SP3 (Specificity protein 3, Transcription factor Sp3) is a member of the Specificity Protein/Krüppel-like Factor (SP/KLF) family of DNA-binding transcription factors, which play a variety of important roles in cell function and homeostasis (1). There are nine known SP transcription factors (SP1-9); each contains a C2H2 zinc finger domain responsible for high affinity binding to GC-rich motifs that enables the activation or repression of target gene expression (2). SP1-4 contain a functional transactivation (TAD) in their amino terminus, which is notably absent from SP5-9. Despite nearly ubiquitous expression of SP transcription factors across most normal cells and tissues, research studies have identified links between expression levels and disease. It was shown, for example, that combined overexpression of v-Myc and SP1/SP3 led to oncogenic transformation of fibroblasts (3). Increased levels of SP transcription factors have likewise been reported in various tumor cells (2), while SP1, SP3, and SP4 were all reported to bind the VEGFR promoter and increase VEGF receptor expression in pancreatic cancer cells (4). The latter observation suggests that SP transcription factors may promote tumor cell growth through multiple mechanisms, including enhancing tumor angiogenesis, providing a potential avenue to therapeutic intervention. Alternate Names DKFZp686O1631; GC-binding transcription factor Sp3; SP3; Sp3 transcription factor; specificity protein 3; SPR-2; SPR2; Transcription factor Sp3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 73, 75, 115, 120 SOURCE: Rabbit