CST, 94103S, ZBTB32 (F5O1L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZBTB32 mouse. Validated for Western Blotting,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, ZBTB32 (F5O1L) Rabbit Monoclonal Antibody (CST #94103) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity ZBTB32 (F5O1L) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZBTB32 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse ZBTB32 protein. Background Zinc finger and BTB domain-containing 32 (ZBTB32), also known as testis zinc finger protein (TZFP) and repressor of GATA3 (ROG), is a transcription factor that belongs to the BTB/POZ-ZF protein family and was first identified in immune cell development and tumorigenesis (1,2). These transcription factor family members contain an N-terminal BTB/POZ domain, which mediates dimerization, and a C-terminal domain with a variable number of zinc fingers that bind DNA (3). ZBTB32 plays a role in T cell homeostasis and inhibits the development of Th2 cells by repressing GATA3 (4,5), and is required for the regulation of CD8 T cells in response to viral infection (6). ZBTB32 is highly expressed by a subset of human and mouse memory B cells but not by naïve B cells (7,8), and is essential for the proliferative burst of natural killer (NK) cells (9). Alternate Names 4930524C15Rik; FAXF; FAZF; GATA repressor; OTTMUSP00000026619; PLZP; Repressor of GATA; Rog; Testis zinc finger protein; Tzf; Tzfp; ZBT32; Zbtb32; zinc finger and BTB domain containing 32; Zinc finger and BTB domain-containing protein 32 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG