CST, 9411S, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100)

Monoclonal Antibody for studying . Validated for WB,IP,IHC,IF,IF,F,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (CST #9411) is ready to ship. Product Usage Information The phosphorylated form of the peptide can be detected with Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) #9411. Sample kinase protocol is attached. Western Blotting: 1:2000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:2400 - 1:9600 Immunofluorescence (Frozen): 1:1600 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:1600 - 1:6400 Peptide ELISA (DELFIA): 1:4000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 80379 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides . Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1