CST, 94127S, CD8 beta (D7C8H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD8B mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD8 beta (D7C8H) Rabbit Monoclonal Antibody (CST #94127) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD8 beta (D7C8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD8β protein. This antibody detects an 11 kDa band of unknown origin in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD8β recombinant protein. Background Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen-Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR-antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). Alternate Names CD8 antigen, beta chain 1; Cd8b; Cd8b1; Ly-; Ly-3; Ly-C; Lymphocyte antigen 3; Lyt-; Lyt-3; Lyt3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 27-32 Source/Isotype: Rabbit IgG