CST, 9412S, VDAC2 Antibody

Polyclonal Antibody for studying VDAC2. Validated for Western Blotting. Highly specific and rigorously validated in-house, VDAC2 Antibody (CST #9412) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VDAC2 Antibody recognizes endogenous levels of total VDAC2 protein. Based on the amino acid sequences, this antibody is not expected to cross-react with other VDAC isoforms. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the central sequence of human VDAC2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Voltage-dependent anion channel (VDAC), ubiquitously expressed and located in the outer mitochondrial membrane, is generally thought to be the primary means by which metabolites diffuse in and out of the mitochondria (1). In addition, this channel plays a role in apoptotic signaling. The change in mitochondrial permeability characteristic of apoptosis is mediated by Bcl-2 family proteins, which bind to VDAC, altering the channel kinetics (2). Homodimerization of VDAC may be a mechanism for changing mitochondrial permeability and supporting release of cytochrome c (3). In mammalian cells, there are three VDAC isoforms, VDAC1, which is the most widely expressed isoform, as well as VDAC2 and VDAC3 (4,5). Alternate Names epididymis secretory sperm binding protein; FLJ23841; hVDAC2; Outer mitochondrial membrane protein porin 2; POR; VDAC-2; VDAC2; voltage dependent anion channel 2; voltage-dependent anion channel 2; Voltage-dependent anion-selective channel protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30 SOURCE: Rabbit