CST, 9414S, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Alexa Fluor^® 488 Conjugate) (CST #9414) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Alexa Fluor Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with phospho-tyrosine-containing peptides . The antibody was conjugated to Alexa Fluor 488 under optimal conditions with an F/P ratio of 2-6. Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1