CST, 9419S, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Sepharose® Bead Conjugate)

Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Sepharose^® Bead Conjugate) (CST #9419) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity Phospho-Tyrosine Mouse Monoclonal Antibody (P-Tyr-100) (Sepharose Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides. Background Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1