CST, 94205T, Stat5 (D2O6Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STAT5A/STAT5B. Validated for WB,WB,IP,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, Stat5 (D2O6Y) Rabbit Monoclonal Antibody (CST #94205) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 55929 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity Stat5 (D2O6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total Stat5 protein. This antibody recognizes both alpha and beta isoforms of Stat5 (Stat5a and Stat5b). Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human Stat5 protein. Background Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Alternate Names epididymis secretory sperm binding protein; GHISID2; MGF; Signal transducer and activator of transcription 5A; Signal transducer and activator of transcription 5B; STA5A; STA5B; STAT5; STAT5A; STAT5B; transcription factor STAT5B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG