CST, 94244T, Bub1 (F5L7L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Bub1. Validated for WB,WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Bub1 (F5L7L) Rabbit Monoclonal Antibody (CST #94244) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:6400 - 1:12800 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Bub1 (F5L7L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Bub1 protein. This antibody does not detect Bub1b protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Bub1 protein. Background Bub1 is a conserved mitotic serine/threonine-protein kinase that plays a role in activating the spindle assembly checkpoint (SAC), which delays progression into anaphase by inhibiting the anaphase-promoting complex/cyclosome (APC/C) until all chromosome kinetochores are attached to the mitotic spindle. Bub1 also plays a role in chromosome segregation (1,2). Bub1 is overexpressed in multiple human cancers, and its expression is predictive of response to cancer therapies (3). Inhibition of Bub1 sensitizes cancer cells to chemotherapy, radiation, and DNA damage response (DDR) inhibitors (4-6), and reverses taxane resistance in prostate cancer cells (7). Alternate Names BUB1; BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1 mitotic checkpoint serine/threonine kinase; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; budding uninhibited by benzimidazoles 1 homolog (yeast); hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG