CST, 9429T, Cleaved Caspase-8 (Asp387) Antibody

Polyclonal Antibody for studying Casp8 (Asp387) cleaved mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-8 (Asp387) Antibody (CST #9429) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved Caspase-8 (Asp387) Antibody detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to the Asp387 cleavage site of rodent caspase-8. Antibodies were purified by protein A and peptide affinity chromatography. Background Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Alternate Names Casp; CASP-8; Casp8; caspase 8; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; Fas-linked ICE-like protease; FLI; FLICE; MAC; MACH; Mch5 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 18, 43 SOURCE: Rabbit