CST, 94350S, NLK (D9X3C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLK. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NLK (D9X3C) Rabbit Monoclonal Antibody (CST #94350) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NLK (D9X3C) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NLK protein. Background Nemo-like kinase (NLK ) is a serine/threonine-protein kinase that regulates multiple signaling pathways, including Wnt/β-catenin, TGFβ, IL-6, and Notch (1-4). NLK contributes to cell proliferation, differentiation, cell fate determination during early embryogenesis and nervous system development in vertebrates (5-7). Recent studies showed that NLK is aberrantly expressed in various types of cancer where it regulates cancer cell proliferation, migration, invasion and survival (8-11). NLK is localized predominantly in nucleus and at a lower level in cytoplasm(12). Homodimerization of NLK is required for its activation and nuclear localization. NLK is activated via intermolecular autophosphorylation at Thr286 (13). NLK interacts with and phosphorylates a number of transcription factors including FOXO1, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4, and LEF-1/TCF (14-18). NLK also associates with E3 ubiquitin ligase NARF and Raptor and regulates their function (19,20). Alternate Names DKFZp761G1211; FLJ21033; LAK1; nemo like kinase; Nemo-like kinase; NLK; Protein LAK1; Serine/threonine-protein kinase NLK Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG