CST, 94396S, KCTD5 Antibody

Polyclonal Antibody for studying KCTD5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, KCTD5 Antibody (CST #94396) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KCTD5 Antibody recognizes endogenous levels of total KCTD5 protein. In some cell lines, this antibody also recognizes a 100 kDa band of unknown origin. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg38 of human KCTD5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background BTB/POZ domain-containing protein KCTD5 is a member of the potassium channel tetramerization domain family of proteins that play a role in transcriptional repression, cytoskeletal regulation, and ion channel gating (1). KCTD5 interacts with bound ubiquitin proteins and cullin3, and acts as a dependent E3 ligase substrate adaptor through interactions with its BTB domain (2). KCTD5 has been identified as a negative regulator of the AKT pathway by acting as an off switch for G-protein coupled receptor signaling by triggering proteolysis of Gβγ heterodimers dissociated from the Gα subunit (3). Alternate Names BTB/POZ domain-containing protein KCTD5; FLJ20040; KCTD5; potassium channel tetramerisation domain containing 5; potassium channel tetramerization domain containing 5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 SOURCE: Rabbit