CST, 94403S, NeuN (E4M5P) Mouse Monoclonal Antibody

Monoclonal Antibody for studying NeuN. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, NeuN (E4M5P) Mouse Monoclonal Antibody (CST #94403) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:400 - 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 93972 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity NeuN (E4M5P) Mouse Monoclonal Antibody recognizes endogenous levels of total NeuN protein. This antibody also recognizes a non-specific band of unknown origin at 140 kDa. Species cross-reactivity for immunofluorescence is mouse only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NeuN protein. Background Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, or dentate nucleus neurons (1). This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS analysis, NeuN was later identified as the gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator (2). Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development (3). Alternate Names FLJ56884; FLJ58356; fox-1 homolog C; Fox-3; FOX3; hCG_1776007; hexaribonucleotide binding protein 3; HRNBP3; NEUN; NeuN antigen; neuronal nuclei; Neuronal nuclei antigen; RBFOX3; RFOX3; RNA binding fox-1 homolog 3; RNA binding protein fox-1 homolog 3; RNA binding protein, fox-1 homolog 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 46-55 Source/Isotype: Mouse IgG2a kappa