CST, 94435SF, Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PCNA (Lys164) ubiquitinate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #94435) is ready to ship. Product Usage Information This product is the carrier free version of product #13439. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13439 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein. Background Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation. PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5). Alternate Names ATLD2; Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 44 Source/Isotype: Rabbit IgG