CST, 94578SF, Siglec-1/CD169 (E1D7G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SIGLEC1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Siglec-1/CD169 (E1D7G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #94578) is ready to ship. Product Usage Information This product is the carrier free version of product #82470. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 82470 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Siglec-1/CD169 (E1D7G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Siglec-1/CD169 protein. This antibody does not cross-react with human Siglec-1/CD169 protein. Non-specific staining was observed in mouse pancreas and testis by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1229 of mouse Siglec-1/CD169 protein. Background Sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169) is a type-I transmembrane glycoprotein and a member of the siglec family of sialic acid-binding proteins. Structurally, Siglec-1 contains a heavily glycosylated extracellular domain and a short intracellular domain with no canonical signaling motifs (1). Expression of Siglec-1 is largely restricted to tissue-resident macrophages found in the bone marrow, liver, spleen, and lymph nodes. Research studies have shown that Siglec-1 is an adhesion molecule and binds to sialylated glycoconjugates to facilitate cell-cell and cell-matrix interactions (2-4). Siglec-1 has also been shown to facilitate the host innate immune response by binding to sialylated epitopes on the surface of viral and bacterial pathogens prior to initiating their phagocytosis (5,6). In the context of cancer, Siglec-1 has been shown to serve as a marker for subsets of proinflammatory macrophages in the tumor microenvironment that aid in the anti-tumor response by phagocytosing tumor cells and recruiting cytotoxic lymphocytes (7). Alternate Names Cd169; OTTMUSP00000016700; Sa; SER; Sheep erythrocyte receptor; sialic acid binding Ig-like lectin 1, sialoadhesin; Sialic acid-binding Ig-like lectin 1; Sialoadhesin; Sigle; Siglec-1; Siglec1; Sn Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 220 Source/Isotype: Rabbit IgG