CST, 94642SF, SirT1 (1F3) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SirT1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, SirT1 (1F3) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #94642) is ready to ship. Product Usage Information This product is the carrier free version of product #8469. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8469 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SirT1 (1F3) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SirT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein representing the central region of human SirT1 protein. Background The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is , which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 ; however, the function of these phosphorylation sites has not yet been determined (9). Alternate Names 75SirT1; hSIR2; hSIRT1; NAD-dependent deacetylase sirtuin-1; NAD-dependent protein deacetylase sirtuin-1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR1; SIR2; sir2-like 1; SIR2-like protein 1; SIR2alpha; SIR2L1; SIRT1; SirtT1 75 kDa fragment; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae); sirtuin 1; sirtuin type 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Mouse IgG1