CST, 9464L, Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody

Polyclonal Antibody for studying FoxO3a (Thr32) phosphate/FoxO1a (Thr24) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody (CST #9464) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody detects endogenous levels of Fox01/Fox03a only when phosphorylated at threonine 24 of Fox01 or threonine 32 of Fox03a. This antibody cross-reacts with phosphorylated Fox04 at threonine 28, but not with Fox01 family members phosphorylated at other sites. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04. Antibodies are purified by protein A and peptide affinity chromatography. Background The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). Alternate Names AF6q21; AF6q21 protein; DKFZp781A0677; FKH1; FKHR; FKHRL1; FKHRL1P2; forkhead box O1; forkhead box O1A (rhabdomyosarcoma); forkhead box O3; forkhead box O3A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; forkhead homolog in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO1; FOXO1A; FOXO2; FOXO3; FOXO3A; MGC12739; MGC31925 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 to 82, 95 SOURCE: Rabbit