CST, 94704S, LYPLA1/APT1 (F7H3F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LYPLA1/APT1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, LYPLA1/APT1 (F7H3F) Rabbit Monoclonal Antibody (CST #94704) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity LYPLA1/APT1 (F7H3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LYPLA1/APT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly155 of human LYPLA1/APT1 protein. Background Protein palmitoylation and depalmitoylation cycling is suggested to have an important role in diabetes pathophysiology. Lysophospholipase 1 (LYPLA1), also known as acyl-protein thioesterase 1 (APT1), catalyzes protein depalmitoylation. Hyperglycemia was shown to reduce the enzymatic activity of LYPLA1/APT1 in human islets. LYPLA1/APT1 deficiency leads to insulin hypersecretion. Furthermore, LYPLA1/APT1 deficiency induces pancreatic β cell failure in mice fed with a high-fat diet (1). In addition, the autophagy receptor SQSTM1/p62 undergoes reversible S-acylation catalyzed by zinc-finger Asp-His-His-Cys S-acyltransferase 19 (ZDHHC19) and LYPLA1/APT1, respectively. -acylation of SQSTM1/p62 by ZDHHC19 enhances degradation of ubiquitinated proteins via autophagy. LYPLA1/APT1 deacylates SQSTM1/p62, suppressing SQSTM1/p62-mediated selective autophagy (2,3). Alternate Names Acyl-protein thioesterase 1; acyl-protein thioesterase-1; APT-1; APT1; hAPT1; LPL-I; LPL1; LYPA1; LYPLA1; Lysophospholipase 1; Lysophospholipase I; lysophospholipid-specific lysophospholipase; LYSOPLA; LysoPLA I Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG