CST, 94725S, KCC2 (D1R2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KCC2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, KCC2 (D1R2R) Rabbit Monoclonal Antibody (CST #94725) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56440 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity KCC2 (D1R2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total KCC2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala970 of human KCC2 protein. Background The potassium/chloride cotransporter 2 (KCC2, SLC12A5) is a neuron-specific transport protein responsible for regulating the cotransport of potassium and chloride ions. KCC2 uses the energy of the electrochemical potassium gradient to export chloride ions from cells, therefore maintaining intracellular chloride ion concentrations in mature neurons (1,2). The intracellular concentration of chloride ions determines the neuronal response to the inhibitory neurotransmitter GABA and glycine. As a result, KCC2 can play a critical role in regulating neuronal excitability in mature central nervous system neurons (3-5). Altered KCC2 expression and reduced KCC2 activity can result in an increase in intracellular chloride ion concentrations and subsequent hyperexcitability of neuronal systems. Cases of aberrant KCC2 function are associated with neurological disorders, such as multiple forms of epilepsy, neuropathic pain, and schizophrenia (6-10). Alternate Names EIEE34; EIG14; Electroneutral potassium-chloride cotransporter 2; erythroid K-Cl cotransporter 2; hKCC2; K-Cl cotransporter 2; KCC2; KIAA1176; Neuronal K-Cl cotransporter; S12A5; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; Solute carrier family 12 member 5 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130-260 Source/Isotype: Rabbit IgG