CST, 9472S, FoxO4 Antibody

Polyclonal Antibody for studying FOXO4. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxO4 Antibody (CST #9472) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity The FoxO4 Antibody detects endogenous levels of Fox04. This antibody is sensitive to phosphorylation within the antigen and preferrentially detects unphosphorylated Fox04. This antibody does not recognize Fox04b. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser71 of human Fox04a. Antibodies are purified by protein A and peptide affinity chromatography. Background The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). Alternate Names AFX; AFX1; Fork head domain transcription factor AFX1; forkhead box O4; Forkhead box protein O4; FOXO4; MGC120490; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit