CST, 94741S, TRAF5 (F6W4N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRAF5 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TRAF5 (F6W4N) Rabbit Monoclonal Antibody (CST #94741) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TRAF5 (F6W4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRAF5 protein. This antibody detects a 40 kDa, 140 kDa, and 220 kDa protein of unknown identity in some cell lines. Non-specific cytoplasmic staining of vascular smooth muscle in mouse lung was observed by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of human tissues. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu39 of mouse TRAF5 protein. Background TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses. TRAF5 regulates signaling through binding to the cytoplasmic domains of TNFR family members including CD40, CD27, CD30, OX40, and lymphotoxin-β receptor (5-10). Overexpression of TRAF5 induces NF-κB activation. Cytoplasmic aggregates of TRAF5, as well as TRAF2, were reported in Hodgkin-Reed-Sternberg cells, resulting in constitutive NF-κB activation (11). Studies of TRAF5-deficient mice suggest that it plays an important role in limiting Th2 immune responses that trigger T-cell-mediated inflammatory diseases and asthma (12). Further studies indicate that TRAF5 binds to the IL-6 receptor gp130 and negatively controls Th17 differentiation (13). In B-cells, TRAF5 negatively regulates toll-like receptor (TLR) mediated cytokine and antibody production (14). Alternate Names TNF receptor-associated factor 5; Traf5 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 64 Source/Isotype: Rabbit IgG