CST, 9477S, Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab® Rabbit Monoclonal Antibody mix

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab^® Rabbit Monoclonal Antibody mix (CST #9477) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Peptide ELISA (DELFIA): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab Species Reactivity: All Species Expected Source / Purification MultiMab rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif. Background Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators. Specification REACTIVITY: All SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG