CST, 9478S, Cleaved Drosophila ICE (drICE) (Asp230) Antibody

Polyclonal Antibody for studying ICE (Asp230) cleaved fruit fly. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cleaved Drosophila ICE (drICE) (Asp230) Antibody (CST #9478) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved Drosophila ICE (drICE) (Asp230) Antibody recognizes endogenous levels of the large 21 kDa fragment of cleaved drICE. This antibody also detects low levels of full length drICE (35 kDa). Species Reactivity: D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp230 of Drosophila ICE protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Cell death in the fruit fly is regulated by many of the same stimuli as mammalian cell death (1). The genome contains seven caspase genes; three encode initiator caspases and four encode effector caspases (reviewed in 2). drICE is a cysteine protease that cleaves baculovirus p35 and lamin DmO and acts downstream of rpr (3). drICE is proteolytically processed during apoptosis into active p21 and p12 subunits. Comparison of the activity between drICE and Dcp-1 has shown that drICE is a more effective inducer of apoptosis than Dcp-1, which plays a role in determining the rate of cell death (4). Alternate Names CASP7; Caspase; Caspase subunit p12; Caspase subunit p21; CG7788; drICE Specification REACTIVITY: Dm SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit