CST, 94817T, Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying luciferase firefly. Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody (CST #94817) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody recognizes exogenously expressed proteins containing firefly luciferase ( Species Reactivity: All Species Expected Source / Purification This recombinant chimeric antibody is engineered from Firefly Luciferase (E9W9V) Rabbit mAb #25039 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, Firefly Luciferase (E9W9V) Rabbit mAb #25039, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase ( ) protein. Background Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, , that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg ) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase ( ) protein or its enzymatic activity (2,3). Alternate Names luciferin 4-monooxygenase Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse chimeric IgG2a