CST, 94886T, WIP1 (E2X1I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WIP1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, WIP1 (E2X1I) Rabbit Monoclonal Antibody (CST #94886) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity WIP1 (E2X1I) Rabbit Monoclonal Antibody recognizes endogenous levels of total WIP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly383 of human WIP1 protein. Background Wild-type p53 induced phosphatase 1 (WIP1)/protein phosphatase magnesium-dependent 1 delta (ppm1d) is a member of the PP2C family of serine/threonine protein phosphatases. WIP1 was initially identified as a p53 target gene, induced in response to ionizing radiation (1). Studies have shown that WIP1 is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (reviewed in 2,3). WIP1 functions in returning cells to a homeostatic state following DNA damage (4,5), as well as in maintaining p53-dependent homeostasis under non-stressed conditions (6). Researchers have shown that increased expression of WIP1 is associated with poor prognosis and lower survival rate in some human cancers (7,8). In contrast, overexpression of WIP1 in p53-negative tumor cells sensitizes them to chemotherapy-induced apoptosis while protecting normal tissue during treatment (9). Alternate Names IDDGIP; JDVS; p53-induced protein phosphatase 1; PP2C-delta; PPM1D; Protein phosphatase 1D; protein phosphatase 1D magnesium-dependent, delta isoform; protein phosphatase 2C delta isoform; Protein phosphatase 2C isoform delta; Protein phosphatase magnesium-dependent 1 delta; protein phosphatase Wip1; protein phosphatase, Mg2+/Mn2+ dependent 1D; protein phosphatase, Mg2+/Mn2+ dependent, 1D; wild-type p53-induced phosphatase 1; WIP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 79 Source/Isotype: Rabbit IgG