CST, 94903S, CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying CD93. Validated for Immunohistochemistry (Paraffin),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #94903) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Live): 1:400 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin), Flow Cytometry (Live) Specificity / Sensitivity CD93/C1qR (E5H9B) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD93/C1qR protein. Background CD93, also known as complement component C1q receptor (C1qR), is a type-I transmembrane glycoprotein belonging to the CLEC14 family of surface antigens, which share a similar ectodomain architecture highlighted by the presence of a C-type lectin domain and multiple EGF-like domains. CD93/C1qR expression is primarily restricted to endothelial cells associated with normal and tumor-associated vasculature as well as immune cells of the myeloid lineage, including monocytes and neutrophils (1). The extracellular domain of CD93/C1qR is rich in O-linked glycosylation modifications, which stabilizes its surface expression by protecting it from proteolytic cleavage (2,3). CD93/C1qR regulates various aspects of endothelial cell biology, including proliferation, migration, and adhesion (4). Indeed, research studies have demonstrated that surface-expressed CD93/C1qR binds to an endothelial-specific extracellular matrix ligand, multimerin-2, and disrupting the interaction compromises angiogenesis (5). Furthermore, CD93 expression is upregulated in tumor vasculature and correlates with poor survival (6-8). Alternate Names C1q receptor 1; C1q/MBL/SPA receptor; C1qR; C1qR(p); C1QR1; C1qRp; CD93; CD93 antigen; CD93 molecule; CDw93; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; Complement component C1q receptor; dJ737E23.1; ECSM3; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG