CST, 9492S, Caspase-7 Antibody

Polyclonal Antibody for studying Casp7. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-7 Antibody (CST #9492) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-7 Antibody detects endogenous levels of both full length caspase-7 (35 kDa) and the large fragment of cleaved caspase-7 following cleavage at aspartic acid 198 (20 kDa). This antibody does not recognize other caspases. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography. Background Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5). Alternate Names Apoptotic protease Mch-3; CASP-7; CASP7; caspase 7; caspase 7 isoform delta; caspase 7, apoptosis-related cysteine peptidase; caspase 7, apoptosis-related cysteine protease; Caspase-7; Caspase-7 subunit p11; Caspase-7 subunit p20; caspase7; CMH-1; ICE-LAP3; ICE-like apoptotic protease 3; LICE2; Lice2 alpha/beta/gamma; MCH3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 20, 35 SOURCE: Rabbit