CST, 9499T, APC6 (D8D8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDC16. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, APC6 (D8D8) Rabbit Monoclonal Antibody (CST #9499) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity APC6 (D8D8) Rabbit Monoclonal Antibody recognizes endogenous levels of total APC6 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with either APC8/CDC23 or APC3/CDC27. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC6 protein. Background Eukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC/C function relies on multiple cofactors, including an APC/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1/FZR1. The CDC20/Cdh1 coactivator is responsible for recognition of APC/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9). Anaphase-promoting complex subunit 6 (APC6, CDC16) is a component of the tetratricopeptide repeat (TPR) sub-complex of the APC/C, which includes APC8/CDC23 and APC3/CDC27. This sub-complex may play an important role in the recruitment of the APC/C activators, CDC20 and Cdh1 (10). Additional evidence suggests that phosphorylation of APC6 and the other TPR subunits during mitosis plays a functional role in regulating the association between TPR subunits and substrate recognition subunits such as Cdc20 (11). Alternate Names ANAPC6; Anaphase-promoting complex subunit 6; anaphase-promoting complex, subunit 6; APC6; CDC16; CDC16 homolog; CDC16Hs; cell division cycle 16; cell division cycle 16 homolog; cell division cycle 16 homolog (S. cerevisiae); Cell division cycle protein 16 homolog; CUT9; Cyclosome subunit 6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG