CST, 95002W2, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying ZAP70 (Tyr319) phosphate/Syk (Tyr352) phosphate. Highly specific and rigorously validated in-house, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #95002) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Background The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Alternate Names 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; DKFZp313N1010; FLJ17670; FLJ17679; FLJ25043; FLJ37489; IMD48; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SRK; STCD; STD; SYK; Syk-related tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG