CST, 95008SF, mGluR1 (D5H10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying mGluR1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, mGluR1 (D5H10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #95008) is ready to ship. Product Usage Information This product is the carrier free version of product #12551. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12551 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity mGluR1 (D5H10) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total mGluR1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu1105 of human mGluR1 protein. Background Metabotropic glutamate receptor 1 (mGluR1) is a G protein-coupled receptor (GPCR) for the neurotransmitter glutamate in the mammalian brain. Unlike ionotropic receptors, metabotropic receptors do not form an ion channel pore themselves but are indirectly linked to ion channels (1). Both mGluR1 and mGluR5 are coupled to phospholipase C and activate inositol phospholipid metabolism via G protein-mediated mechanisms. Upon phosphatidylinositol activation, the second messenger calcium is released and generates a calcium-activated chloride current. Metabotropic glutamate receptors other than mGluR1 and mGluR5 inhibit adenylate cyclase (1-3). mGluR1 does not share sequence homology with conventional GPCRs (1). mGluR1 forms a homodimer and is linked to synaptic plasticity, as well as long-term potentiation and long-term depression. Furthermore, mGluR1 is a potential therapeutic target for various psychiatric and neurological diseases, including schizophrenia, epilepsy, and Parkinson and Alzheimer diseases (4-6). Alternate Names glutamate metabotropic receptor 1; glutamate receptor, metabotropic 1; GPRC1A; GRM1; GRM1A; Metabotropic glutamate receptor 1; mGlu1; MGLUR1; MGLUR1A; PPP1R85; protein phosphatase 1, regulatory subunit 85; SCA44; SCAR13 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 145, >300 Source/Isotype: Rabbit IgG