CST, 9507S, Cleaved Caspase-9 (Asp353) Antibody

Polyclonal Antibody for studying Casp9 (Asp353) cleaved rat. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cleaved Caspase-9 (Asp353) Antibody (CST #9507) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cleaved Caspase-9 (Asp353) Antibody detects endogenous levels of the large fragment (17 kDa or 38 kDa with prodomain) of caspase-9 resulting from cleavage at aspartic acid 353. This antibody does not recognize full length caspase-9 or any other cleaved caspases. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp353 of rat caspase-9. Antibodies are purified by protein A and peptide affinity chromatography. Background Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). Alternate Names 25 kDa caspase-9 dominant negative protein; Apaf3; apoptotic protease activating factor 3; apoptotic protease Mch-6; Casp-9-CTD; Casp9; Casp9_v1; caspase 9, apoptosis-related cysteine peptidase; Caspase 9, isoform CRA_a; Caspase-9; caspase-9 dominant negative form; Caspase-9 long isoform; caspase-9-carboxyl-terminal divergent; Ice-Lap6; Ice-like apoptotic protease 6; Mch6; RNCASP9 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 17, 38 SOURCE: Rabbit