CST, 95084T, CELF1 (E8Q4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CELF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CELF1 (E8Q4G) Rabbit Monoclonal Antibody (CST #95084) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting Specificity / Sensitivity CELF1 (E8Q4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CELF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro252 of human CELF1 protein. Background CELF1, also known as CUG-BP1, is a member of the CUG-BP, Elav-like family (CELF), which can bind enhancers to direct alternate splicing (1). CELF1 is widely expressed, can bind triple CUG repeat sequences, and was initially shown to play a role in alternative translation of C/EBP isoforms. (2-4). CELF1 plays an important role in the pathogenesis of myotonic dystrophy, a disease categorized by expansion of CTG repeats in the myotonia protein kinase gene ( ). This kinase is capable of phosphorylating CELF1, which can regulate its localization and affect mRNA processing and transport (2,3). In addition, CELF1 has also been shown to play roles in various cancer types (5-7). Alternate Names 50 kDa nuclear polyadenylated RNA-binding protein; bruno-like 2; Bruno-like protein 2; BRUNOL2; CELF-1; CELF1; CUG RNA-binding protein; CUG triplet repeat RNA-binding protein 1; CUG triplet repeat, RNA binding protein 1; CUG triplet repeat, RNA-binding protein 1; CUG-BP; CUG-BP- and ETR-3-like factor 1; CUG-BP1; CUGBP; CUGBP Elav-like family member 1; CUGBP, Elav-like family member 1; CUGBP1; Deadenylation factor CUG-BP; EDEN-BP; EDEN-BP homolog; embryo deadenylation element binding protein; Embryo deadenylation element-binding protein homolog; hNab50; NAB50; NAPOR; nuclear polyadenylated RNA-binding protein, 50-kD; RNA-binding protein BRUNOL-2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG