CST, 9508S, Caspase-9 (C9) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Casp9. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Caspase-9 (C9) Mouse Monoclonal Antibody (CST #9508) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10068 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Caspase-9 (C9) Mouse Monoclonal Antibody detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal Antibody is produced by immunizing mice with a recombinant human caspase-9 protein. Background Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10). Alternate Names APAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 47/37/35 (H). 49/39/37 (M). 51/40/38 (R). Source/Isotype: Mouse IgG1