CST, 95109S, Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying perilipin 2. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody (CST #95109) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 79979 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Perilipin-2 (E6G6M) Rabbit Monoclonal Antibody recognizes endogenous levels of total perilipin-2 protein. This antibody does not cross-react with perilipin-1 protein. Non-specific staining was observed in brain by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human perilipin-2 protein. Background Eukaryotic cells store lipids in lipid droplets. These intracellular lipid storage structures are covered by members of the perilipin family of lipid droplet coat proteins. Perilipins establish an interface between stored lipids and the cytosol, and coordinate lipid storage and utilization (1-3). Mice deficient in perilipin-2 have decreased levels of triglycerides in the liver and are protected from fatty liver disease. In addition, these mice show increased autophagy in the liver. In contrast, overexpression of perilipin-2 protects lipid droplets from autophagy (4). Alternate Names ADFP; Adipophilin; Adipose differentiation-related protein; ADRP; MGC10598; perilipin 2; Perilipin-2; PLIN2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG