CST, 95128S, PPAR gamma (D8I3Y) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PPAR-gamma. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PPAR gamma (D8I3Y) Mouse Monoclonal Antibody (CST #95128) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PPAR gamma (D8I3Y) Mouse Monoclonal Antibody recognizes endogenous levels of total PPARγ protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His494 of human PPARγ protein. Background Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5). Alternate Names CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxisome proliferator activated receptor gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 53, 57 Source/Isotype: Mouse IgG1