CST, 95153T, Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Beta6. Validated for WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody (CST #95153) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:3200 - 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 52976 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Integrin beta6 (E4M9P) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin β6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human integrin β6 protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and eight β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling) but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). The avβ6 subunit was identified in epithelial cells and interacts with the fibronectin receptor integrin α5 (3,4). It is expressed during development, but expression becomes repressed in adulthood (5). It is involved in wound healing, fibrosis, pulmonary fibrosis in particular, and tumorigenesis (6-8). When the dimer is unregulated in the membrane in response to injury, the integrin β6 subunit binds directly to an RGD motif present in fibronectin and TGF-β, both present in the extracellular matrix. Integrin β6 cleaves and binds the LAP fragment of latent TGF-β, thereby releasing active TGF-β into the ECM to activate myofibroblasts, which consequently mediate ECM remodeling (6-8). Alternate Names AI1H; Integrin beta-6; integrin subunit beta 6; integrin, beta 6; ITB6; ITGB6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105 Source/Isotype: Rabbit IgG