CST, 95197T, MTA2 (F2D5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTA2. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, MTA2 (F2D5U) Rabbit Monoclonal Antibody (CST #95197) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:200 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity MTA2 (F2D5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTA2 protein. This antibody does not cross-react with MTA1 and MTA3 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro602 of human MTA2 protein. Background MTA2 is a component of the NuRD deacetylase complex, which is recruited by MBD3 and modulates the enzymatic activity of the histone deacetylase core (1,2). MTA2 is highly related to MTA1, but both proteins exist in biologically distinct complexes, with MTA2 being associated with complexes containing Sin3a and CHD4 (3) MTA2 has been shown to bind ERα and repress its activity and contributes to estradiol- and tamoxifen-resistant breast cancers (4). Indeed, the MTA proteins are critical regulators of many biological functions in both cancer and non-cancer settings (5,6). MTA2 upregulation has been reported in a multitude of cancers, including hepatocellular carcinomas, colorectal cancers, gastric cancers, and esophageal cancers (7-10). MTA2 has also been described in B cell development, where it interacts with Aiolos and Ikaros to control target genes (11). Alternate Names DKFZp686F2281; metastasis -associated gene 1-like 1; metastasis associated 1 family member 2; metastasis associated 1 family, member 2; metastasis associated gene family, member 2; Metastasis-associated 1-like 1; metastasis-associated protein 2; Metastasis-associated protein MTA2; MTA1-L1 protein; MTA1L1; MTA2; p53 target protein in deacetylase complex; PID Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG