CST, 95203SF, Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Basigin/EMMPRIN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #95203) is ready to ship. Product Usage Information This product is the carrier free version of product #13287. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13287 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total EMMPRIN protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala254 of human EMMPRIN protein. Background Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily (1). Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed (2). Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression (2-4). Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy (5,6). A recent study identified the gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition (7). Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells (8), and as an obligate receptor for the malarial parasite on human erythrocytes (9). Alternate Names 5F7; BASI; Basigin; basigin (Ok blood group); BSG; CD147; CD147 antigen; Collagenase stimulatory factor; EMMPRIN; EMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK; OK blood group antigen; SLC7A11; TCSF; Tumor cell-derived collagenase stimulatory factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38-58 Source/Isotype: Rabbit IgG