CST, 95231S, UQCRFS1/RISP Antibody

Polyclonal Antibody for studying RISP. Validated for Western Blotting. Highly specific and rigorously validated in-house, UQCRFS1/RISP Antibody (CST #95231) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UQCRFS1/RISP Antibody recognizes endogenous levels of total UQCRFS1/RISP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro86 of human UQCRFS1/RISP protein. Antibodies are purified by peptide affinity chromatography. Background Ubiquinol-cytochrome c reductase iron-sulfur subunit (UQCRFS1), also known as Rieske iron-sulfur protein (RISP), is a component of Complex III in the mitochondrial electron transport chain. UQCRFS1/RISP and two other subunits, cytochrome b (MT-CYB) and cytochrome c1 (CYC1), are essential for the catalytic activity of Complex III (1). Studies show that UQCRFS1/RISP is required for fetal mouse hematopoietic stem cell differentiation and adult mouse hematopoietic stem cell quiescence (2). In addition, loss of UQCRFS1/RISP decreases the strength of T cell receptor (TCR) signaling, indicating that this protein is critical for optimal TCR signaling (3). Alternate Names 8 kDa subunit 9; Complex III subunit 5; Complex III subunit IX; Cytochrome b-c1 complex subunit 11; Cytochrome b-c1 complex subunit 5; Cytochrome b-c1 complex subunit 9; Cytochrome b-c1 complex subunit Rieske, mitochondrial; epididymis secretory sperm binding protein; MC3DN10; Rieske iron-sulfur protein; Rieske protein UQCRFS1; RIP1; RIS1; RISP; Su9; Subunit 9; Ubiquinol-cytochrome c reductase 8 kDa protein; Ubiquinol-cytochrome c reductase iron-sulfur subunit; ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1; UCRI; UQCR5; UQCRFS1; UQCRFS1 mitochondrial targeting sequence; UQCRFS1 MTS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 23 SOURCE: Rabbit