CST, 95336S, Prdx6 Antibody

Polyclonal Antibody for studying PRDX6. Validated for Western Blotting. Highly specific and rigorously validated in-house, Prdx6 Antibody (CST #95336) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Prdx6 Antibody recognizes endogenous levels of total Prdx6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Prdx6 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Peroxiredoxin 6 (Prdx6) belongs to an antioxidant enzyme family of non-seleno peroxidases (prdx). It is a unique member of the Prdx family exhibiting both glutathione peroxidase and phospholipase A2 activities (1,2). Prdx6 regulates phospholipid turnover as well as protects cells against oxidative injury. Prdx6 is expressed in all major organs with a particularly high level in lung where it regulates lung surfactant phospholipid synthesis and turnover (3-5). Studies show Prdx6 is aberrantly expressed in various cancers and promotes cancer cell metastasis and invasion (6,7). Elevated expression of Prdx6 and other prdx family members contributes to drug resistance in cancer cells (8,9). Prdx6 is also expressed in neutrophils, where it regulates the function of these cells and activates NADPH oxidase (Nox2) ( 10-12). Alternate Names 1-Cys; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; epididymis secretory sperm binding protein Li 128m; Glutathione-dependent peroxiredoxin; HEL-S-128m; KIAA0106; Liver 2D page spot 40; MGC46173; Non-selenium glutathione peroxidase; NSGPx; p29; peroxiredoxin 6; Peroxiredoxin-6; PRDX6; PRX; Red blood cells page spot 12 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit