CST, 95339S, VAPB (E7R3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VAPB. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, VAPB (E7R3Z) Rabbit Monoclonal Antibody (CST #95339) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity VAPB (E7R3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total VAPB protein. This antibody non-specifically labels spermatids in fixed frozen mouse testis by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human VAPB protein. Background The human VAP family proteins, which include VAPA (also known as VAP33), VAPB, and VAPC, were initially identified as homologues of vesicle-associated membrane protein (VAMP)-associated protein (VAP) that is involved in exocytosis of neurotransmitters (1). VAPB is an integral endoplasmic reticulum (ER) protein whose amino terminus projects into the cytosol. A proline to serine substitution at position-56 (VAPBP56S) leads to an autosomal-dominant form of amyotrophic lateral sclerosis (ALS), classified as ALS-8. (2). VAPB has been implicated in a variety of processes, including ER stress and the unfolded protein response (UPR), ER to Golgi transport, and bouton formation at the neuromuscular junction (1,2). The P56S mutation causes almost complete loss of function of VAPB to mediate UPR by inducing its misfolding and localization shift to non-ER compartments (1). A possible role of VAPB as a pathologic marker in PBMCs from ALS patients has been published (3). Alternate Names ALS8; VAMP (vesicle-associated membrane protein)-associated protein B and C; VAMP associated protein B and C; VAMP-associated 33 kDa protein; VAMP-associated protein B; VAMP-associated protein B/C; VAMP-associated protein C; VAMP-B; VAMP-B/VAMP-C; VAMP-C; VAP-B; VAP-B/VAP-C; VAP-C; VAPB; Vesicle-associated membrane protein-associated protein B/C Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG