CST, 95343S, STK33 (D3S4R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STK33. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, STK33 (D3S4R) Rabbit Monoclonal Antibody (CST #95343) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity STK33 (D3S4R) Rabbit Monoclonal Antibody recognizes endogenous levels of total STK33 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro76 of human STK33 protein. Background Serine/threonine protein kinase (STK33) is a 524-residue kinase that belongs to the calcium/calmodulin-dependent family of kinases (1, 2). STK33 exhibits differential expression in normal and malignant cancer tissue, including hepatocellular carcinoma (3). STK33 expression is required in the context of mutant KRAS, the most commonly mutated human oncogene, to maintain for cellular viability and proliferation (4). STK33 depletion via modulation of interacting proteins (e.g., HSP90) results in proteasome-mediated degradation of STK33 in human cancer cells, triggering apoptosis (4). Alternate Names serine/threonine kinase 33; Serine/threonine-protein kinase 33; STK33 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG