CST, 9535T, PDCD4 (D29C6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDCD4. Validated for WB,IP,IHC,IF,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, PDCD4 (D29C6) Rabbit Monoclonal Antibody (CST #9535) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:200 Immunofluorescence (Immunocytochemistry): 1:600 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PDCD4 (D29C6) Rabbit Monoclonal Antibody detects endogenous levels of total PDCD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human PDCD4 protein. Background PDCD4 (programmed cell death protein 4) was identified as a suppressor of neoplastic transformation (1). It interacts with eukaryotic initiation factors eIF4AI and eIF4AII (2). PDCD4 colocalizes with eIF4A in the cytoplasm and inhibits the activity of eIF4A as an ATP-dependent RNA helicase (2). PDCD4 inhibits cap-dependent translation (2). Upon stimulation by mitogens, PDCD4 is phosphorylated on Ser67 by S6K1 kinase and then degraded through the ubiquitin pathway leading to enhanced translation and cell growth (3). Alternate Names H731; MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; PDCD4; programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG