CST, 9536S, Enolase-2 Antibody

Polyclonal Antibody for studying Enolase2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Enolase-2 Antibody (CST #9536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Enolase-2 Antibody detects endogenous levels of total enolase-2 protein and does not corss-react with enolase-1. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human enolase-2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Enolase is a glycolytic enzyme that is involved in the conversion of 2-phosphoglycerate to phosphoenolpyruvate (1). Mammalian enolase has three subunits: α, β, and γ, that can form homo and heterodimers. Homodimers of γ enolase are neuronal-specific (2). Research studies have shown elevated levels of neuro-specific enolase-2 in neuroblastoma (2) and small-cell lung cancer (3,4). Alternate Names 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; Gamma-enolase; HEL-S-279; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; neurone-specific enolase; NSE Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 47 SOURCE: Rabbit