CST, 9546T, Cleaved PARP (Asp214) (19F4) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PARP1 (Asp214, 89 kD) cleaved. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously validated in-house, Cleaved PARP (Asp214) (19F4) Mouse Monoclonal Antibody (CST #9546) is ready to ship. Product Usage Information Western Blotting: 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cleaved PARP (Asp214) (19F4) Mouse Monoclonal Antibody detects endogenous levels of the large fragment (89 kDa) of PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP. Background PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases (2,3) and is one of the main cleavage targets of caspase-3 (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.) Alternate Names ADP-ribosyltransferase (NAD+); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 89 Source/Isotype: Mouse IgG1