CST, 95473T, OMA1 (D4J7K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OMA1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, OMA1 (D4J7K) Rabbit Monoclonal Antibody (CST #95473) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 64306 . Protocol Available protocols: Western Blotting Specificity / Sensitivity OMA1 (D4J7K) Rabbit Monoclonal Antibody recognizes endogenous levels of total OMA1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu504 of human OMA1 protein. Background Mitochondria continuously divide and fuse. This dynamic process is highly regulated in response to various physiological cues (1,2). The GTPase OPA1 mediates the fusion of the mitochondrial inner membrane. Constitutive proteolytic processes mediated by OMA1 (S1 site) and YME1L (S2 site) convert long isoforms (L-OPA1) into short isforms (S-OPA1). The balance between L-OPA1 and S-OPA1 is required to maintain a normal morphology of mitochondria (3,4).OMA1 is synthesized as a precursor and processed into a mature form (5,6). OMA1 is constitutively active and cleaves L-OPA1 at the S1 site. However, various stress stimuli can further activate OMA1 and result in the rapid and complete conversion of L-OPA1 into S-OPA1, which inhibits fusion and causes mitochondrial fragmentation (6). Alternate Names 2010001O09Rik; DAB1; FLJ33782; Metalloendopeptidase OMA1, mitochondrial; metalloprotease related protein 1; Metalloprotease-related protein 1; MPRP-1; MPRP1; OMA1; OMA1 homolog, zinc metallopeptidase; OMA1 homolog, zinc metallopeptidase (S. cerevisiae); OMA1 zinc metallopeptidase; OMA1 zinc metallopeptidase homolog; overlapping activity with M-AAA protease; Overlapping with the m-AAA protease 1 homolog; peptidase; YKR087C; zinc metallopeptidase OMA1; ZMPOMA1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG