CST, 95536S, SARS-CoV-2 Nsp9 Antibody

Polyclonal Antibody for studying nsp9-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp9 Antibody (CST #95536) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Nsp9 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp9 protein in infected cells. In infected Vero-E6 cells, a band of unknown identity was observed at 60 kDa, and a cross-reactive band was observed in both mock and infected Vero-E6 lysates at 35 kDa. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly93 of SARS-CoV-2 Nsp9 protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp9 is a non-structural protein that is derived from ORF1a and ORF1b (2). Although the function of Nsp9 is not completely understood, it is known to be an RNA-binding protein with a unique architecture that interacts with the viral polymerase holoenzyme complex. It is well conserved between coronaviruses and is thought to be essential for viral replication, thus making it a target of therapeutic development (3). Alternate Names non-structural protein 9; non-structural protein 9 (4141-4253); nsp9; nsp9-pp1a-pp1ab; SARS-CoV-2 nsp9 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 10 SOURCE: Rabbit