CST, 95558T, RIF1 (D2F2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RIF1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, RIF1 (D2F2M) Rabbit Monoclonal Antibody (CST #95558) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity RIF1 (D2F2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total RIF1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala400 of human RIF1 protein. Background The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its effect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double-strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5' end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-8). Oct-4 and Smad3 modulate RIF1 expression in mouse embryonic stem cells, and RIF1 may regulate embryonic stem cell stability during cell proliferation (9). Inhibition of ATR or CHK1 activity induces CDK1-mediated phosphorylation of RIF1 at serine 2205 (human)/serine 2153 (mouse), leading to firing of dormant origins of DNA replication during S phase (10,11). Alternate Names DKFZp434D1026; DKFZp781N1478; FLJ12870; RAP1 interacting factor homolog; RAP1 interacting factor homolog (yeast); Rap1-interacting factor 1 homolog; replication timing regulatory factor 1; RIF1; Telomere-associated protein RIF1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 274 Source/Isotype: Rabbit IgG