CST, 95633SF, TEAD1 (D9X2L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TEAD1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, TEAD1 (D9X2L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #95633) is ready to ship. Product Usage Information This product is the carrier free version of product #12292. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12292 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TEAD1 (D9X2L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TEAD1 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe193 of human TEAD1 protein. Background The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of Yes-associated protein (YAP), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). When the Hippo pathway is turned on, YAP is phosphorylated by LATS1/2 kinase and sequestered in the cytoplasm by 14-3-3 protein binding, rendering YAP inactive. When the Hippo pathway is off, non-phosphorylated YAP translocates to the nucleus where it associates with various transcription factors including members of the transcriptional enhancer factor (TEF) family, also known as the TEA domain (TEAD) family (TEAD1-4) (5,6). Although widely expressed in tissues, the TEAD family proteins have specific tissue and developmental distributions. YAP/TEAD complexes regulate the expression of genes involved in cell proliferation and apoptosis (5). Alternate Names AA; atrophia areata, peripapillary chorioretinal degeneration; NTEF-1; Protein GT-IIC; REF1; SV40 transcriptional enhancer factor; TCF-13; TCF13; TEA domain family member 1; TEA domain family member 1 (SV40 transcriptional enhancer factor); TEA domain transcription factor 1; TEAD-1; TEAD1; TEF-1; TEF1; Transcription factor 13; transcriptional enhancer factor 1; Transcriptional enhancer factor TEF-1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG